Comparative Medicine, Copyright 2011
by the American Association for Laboratory Animal Science
Vol 6l,No 3, June 2011, Pages 258-262
Received: 15 Nov 2010/Revision requested: 08 Dec 2010
Accepted: 18 Dec 2010
Hemodynamic and Histologic Characterization of a Swine (Suss crofa domestica) Model of Chronic Pulmonary Arterial Hypertension
Abraham Rothman, Robert G Wiencek, Stephanie Davidson, William N Evans, Humberto Restrepo, Valeri Sarukhanov, Amanda Rivera-Begeman, David Mann
ChildrenŐs Heart Center Nevada, 3006 S Maryland Pkwy, Ste 690, Las Vegas, NV 89109, USA.Email: email@example.com.
The purpose of this work was to develop and characterize an aortopulmonary shunt model of chronic pulmonary hypertensionin swine and provide sequential hemodynamic, angiographic, and histologic data by using an experimental endoarterial biopsy catheter. Nine Yucatan female microswine (Suss crofa domestica) underwent surgical anastomosis of the left pulmonary artery to the descending aorta. Sequential hemodynamic, angiographic, and pulmonary vascular samples were obtained. Six pigs (mean weight, 22.4 ± 5.3 kg; mean age, 7.3 ± 2.7 moat surgery) survived long-term (6 mo) and consistently developed marked pulmonary arterial hypertension. Angiography showed characteristic central pulmonary arterial enlargement and peripheral tortuosity and pruning. The biopsy catheter was safe and effective in obtaining pulmonary endoarterial samples for histologic studies, which showed neointimal and medial changes. Autopsy confirmed severe pulmonary vascular changes, including concentric obstructive neointimal and plexiform-like lesions. This swine model showed hemodynamic, angiographic, and histologic characteristics of chronic pulmonary arterial hypertension that mimicked the arterial pulmonary hypertension of systemic-to-pulmonary arterial shunts inhumans. Experimental data obtained using this and other models and application of an in vivo endoarterial biopsy technique may aid in understanding mechanisms and developing therapies for experimental and human pulmonary arterial hypertension.